Web3.1 Prepare LipidTOX™ Green neutral lipid stain. Dilute the 1000X LipidTOX™ Green neutral lipid stain 1:1000 in buffer to make a 1X solution. A volume of 100 µL/well is required. 3.2 Remove buffer from the cells (after the appropriate number of buffer washes from step 2.6). 3.3 Add 100 µL of 1X LipidTOX™ Green neutral lipid stain to ... WebDec 27, 2024 · Cells were stained with 0.5 μM NIM-7 for 1 h, or HCS LipidTOX™ Deep Red Neutral Lipid Stain (1 : 1000 dilution) for 0.5 h, or 50 nM LysoTracker® Blue DND-22 for 0.5 h, or 50 nM MitoTracker™ …

Different Sensitivity of Macrophages to Phospholipidosis …

WebDec 2, 2024 · Adipocyte lipid droplet staining was performed with HCS LipidTOX™ Deep Red Neutral Lipid Stain (Thermo Fisher, Waltham, MA, USA). After washing cells with PBS three times, the adipocytes were fixed with 4% formaldehyde and incubated for 30 min at room temperature. WebJan 1, 2014 · Here, we provide a detailed protocol to directly detect and quantify accumulation of neutral lipids in vitro, exemplified by staining of human breast and … marian swanson chelsea schumacher

HCS LipidTOX™ Green Neutral Lipid Stain, for cellular …

WebTransmembrane protein 68 (TMEM68) is an endoplasmic reticulum (ER)-anchored acyltransferase family member of unknown function. In the current study we show that overexpression of TMEM68 promotes TG accumulation and lipid droplet (LD) formation in a conserved active sites-dependent manner. WebHcs Lipidtox Green Neutral Lipid Stain Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS … WebThermo Fisher hcs lipidtox deep red neutral lipid stain Figure 2. B, third panel).. Corresponding areas in different images are indicated by blue and purple squares, respectively. Scale bars in (A) and (B), 2.5 μm. natural gas training events